Thread: Peptide Guide

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Peptide Information

Peptides come in the form of lyophilized (freeze dried) powder. The amount of powder/product is stated in International Units (IU's) or in Milligrams (mg).

Melanotan peptides (Afamelanotide & Melanotan 2), PT-141, CJC-1295 & GHRPs (GRHP-2 & GHRP-6), HGH, GH fragments, et cetera use Bacteriostatic Water (BW). Buy Bacteriostatic Water for injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% of benzyl alcohol added as a bacteriostatic preservative. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. The pH is 5.7 (4.5 to 7.0)

Bacteriostatic water 30mL

For IGF use an acetic acid solution (.6%) which is 7 parts distilled water and 1 part vinegar to reconstitute. You must filter the distilled water and white wine vinegar through a sterile 20 micron syringe filter before use. Sodium Chloride (NaCl) is used to buffer the injection.

1.) Take an alcohol swab to the stopper of both your peptide vial and the vial of the dilutent.

2.) Draw your preferred dilutent (BW) with a 1cc syringe. Choose an amount that will make measuring the final product simple.

1ml(cc) per 10 mg vial of Melanotan would mean each 10 tick marks on a U100 slin syringe would equal 1mg of Melanotan
1ml(cc) per 10 IU vial of HGH would mean each 10 tick marks on a U100 slin syringe would equal 1 IU of HGH

3.) Take the syringe with the dilutent and push it into the vial of lyophilized powder letting the dilutent dissolve the peptide. Many (not all) peptides are sealed with vacuum pressure, be careful.

4.) After dilutent has been added to the vial, gentling swirl the vial until the lyophilized powder has dissolved and you are left with a clear liquid. The peptide is now ready for use.

5.) Store your now reconstituted peptide in the refrigerator.
Measurement

After successfully reconstituting your peptide, measure the desired amount out for injection. Use a U100 insulin syringe to draw out and inject your product.

Since you know the amount of IU's/MG's in your vial, we divide this out as follows:
You will need to know the following to be successful: 1ml = 1cc = 100 IU's

We take our dose from the label of the dry lyophilized powder and we divide that into the amount of dilutent used.

example- We used 1cc(ml) of water. We have a 10 IU vial of HGH.
From our formula above we know that 1cc = 100 IU's, so we have 100 IU's of water.
We now divide the 100 IU's (the amount of our water) by 10 IU's (the amount of our HGH)
100 IU / 10 IU = 10

This 10 will perfectly correspond with the markings on a U100 insulin syringe. In our example every 10 mark on our syringe will equal 1 IU of HGH. Want to draw out 2 IU's of GH? ....draw out to the 20 mark on the syringe (1/5th of the syringe).

Say you have a 1mg vial and you add 1ML you get
1000mcg/1mL: 10 mcg per IU
1000mcg/2mL: 5 mcg per IU

Say you have a 10mg vial and you add 1ML you get
10mg/1mL: 1 mg per 10 IU
10mg/2mL: .5 mg per 10 IU

Say you have a 20mg vial and you add 1ML you get
20mg/1mL: 2 mg per 10 IU
20mg/2mL: 1 mg per 10 IU

Say you have a 10iu vial and you add 1ML you get
10iu/1mL: 1 iu per 10 IU (on the syringe - 1/10th the product)
10iu/2mL: 1 iu per 20 IU (on the syringe - still 1/10th the product)
Say you have a 5000iu vial and you add 1ML you get
5000iu/1mL: 500iu per 10 IU
5000iu/2mL: 250iu per 10 IU

Recap:
1.) How much actual product you are dealing with (MG or IU)
2.) How much water (dilutent) you are using to add to powder
3.) Divide the amount of water in units by the amount MG/IU.
4.) This result will equal the measurement on your U100 Insulin syringe per unit
5.) Multiply the number you get it step 4 by how many units you want to inject. This is the number to draw to on your syringe

Growth Hormone Releasing Peptide (GHRP-6)

GHRP-6 (Growth Hormone Releasing Peptide) Dose:

Light: 50mcg
Common: 100mcg
Large: 150mcg

Growth Hormone Releasing Peptide (GHRP-6) is a peptide in the growth factor family. It has strong effect on the release of Human Growth Hormone (HGH) in a specific and dose-related manner. GHRP can be effectively used in the treatment of growth hormone (GH) deficiency. Growth hormone releasing hexapeptide works by signaling the pituitary gland to begin growth hormone secretion.

Increased GH and IGF-1 levels are highly desirable for those looking to improve physique. Human growth hormone has been known to enhance immune response and stimulate the immune system, particularly older subjects. Dosed at night for anti-aging purposes and multiple times throughout the day for anabolism. GHRP is often used in conjunction with GHRH CJC-1295 (GRF 1-29) to amplify growth hormone pulse. Avoid fats and carbohydrate a half hour on each side of dosing GHRP-6.

Bodybuilders and athletes utilize GHRP in an effort to build more muscle and burn fat. Some GHRP-6 users include it in their post cycle therapy (PCT). Cycling GHRP-6 in the off weeks from IGF/GH cycles is also becoming prevalent. Researchers wish to kick-start their body into producing their own natural GH & IGF, while gaining as if they remained on the GH/IGF peptides.

GHRP-6's main use is to promote food intake by stimulating hunger and aid in energy metabolism. The major side effect being a significant increase in appetite due to a stimulating the release of Ghrelin (about 20 minutes post injection), a hormone released naturally in the lining of the stomach and increases hunger and gastric emptying. This is why GHRP-6 can be used in the treatment of cachexia (wasting), eating disorders and obesity.

Benefits of increased HGH levels through GHRP-6 stimulation include: an increase in strength, muscle mass and body fat loss, rejuvenation and strengthening of joints, connective tissue and bone mass. Enhanced HGH secretion also leads to the liver secreting more IGF-1, which is thought to be the primary anabolic mechanism of action for Growth Hormone.

GHRP-6 Peptide: GHRP-6 peptide is typically offered in 5mg vials

5MG GHRP-6 Peptide

Mixing: Bacteriostatic water is used for reconstitution. When diluted, peptide lasts a very long time when left alone in the refrigerator (months)

Example- 2.5ml(cc) bacteriostatic water per 5mg GHRP vial equates to a 100mcg dose approximately each 2-3 marks on a U100 insulin syringe.
Example- 5ml(cc) bacteriostatic water per 5mg GHRP vial equates to a 100mcg dose approximately every 5 marks on a U100 insulin syringe.

Dosing: The saturation dose of GHRP-6 has been determined to be around 100mcg. More is not better in regards to this secretalogue

5mg GHRP = 5,000mcg

5,000mcg/100mcg = 50 100mcg GHRP doses per 5mg

GHRP-6 may be the most cost effective secretalogue available today
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Insulin-like Growth Factor LR3 (IGF-1)
IGF-1 (Long R3 IGF-1) Dose:
Light: 20 mcg
Common: 40 mcg
Large: 80 mcg

IGF-1 is injected post work out (PWO) or in the AM on non workout days for 4-6 week intervals. Do not exceed 100mcg.

Insulin-like Growth Factor (Long-R3 IGF-1), an 83 amino acid analog of IGF-1 is a highly anabolic hormone released primarily in the liver with the stimulus of growth hormone (HGH).

IGF-1 LR3 Molecule

IGF-1 is the most potent growth factor found in the body and causes muscle cell hyperplasisa.

Freeze dried (lyophilized) IGF-1 (in powder state) should be stored in the freezer (-18 degrees celsius).
Example Long r3 IGF-1 kit contains:

1000mcg of lyophilized (freeze dried) Long R3 IGF-1
2 mls of 0.6% Acetic Acid (AA)
30ml Sodium Chloride (NaCL) as buffer

Dilute the IGF-1 peptide with 2mls of Acetic Acid (.6%). Assuming (*DO NOT ASSUME*) Acetic Acid (AA) will yield the correct pH balance of your research peptide.
Note: This creates a concentration of 500mcg/ml. So each 1/10 of a ML is 50mcg’s.

Draw the desired amount of IGF in to a syringe. Desired amount should be the approx dosage wanted.
Example- 2mls AA used to reconstitute IGF-1 1mg vial means 5 units on a U100 insulin syringe would equal 25mcg IGF-1 LR3
Pre-load your syringes at 5iu (25mcg IGF-1). Divide your IGF-1 into 40 syringes for storage in the freezer.
Thaw prior to administration. Draw from your NaCL after thawed to buffer (.5ml is enough).

Unknown whether injecting IGF-1 to increase muscle growth is efficacious. Many believe in the value of this powerful growth factor. Question currently is whether IGF-1 is effective when not manufactured by the tissues themselves.

It is possible to go into hypoglycemia fro IGF-1 supplementation. Effect is dose dependant.
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Growth Hormone
Human Growth Hormone (HGH) Dose:

Month 1: HGH 2 IU one injection every other day
Months 2-4: HGH 3 IU one injection five days a week
Months 5-6: HGH 4 IU one injection five days a week
4-6 month cycles are optimal, year round if you are over age 35.
human growth muscle

Human Growth Hormone (rHGH, HGH, or GH) is a protein molecule consisting of 191 amino acids. Genetically engineered recombinant human growth hormone is completely identical to the growth hormone made by the human pituitary gland. Researchers have proven growth hormone therapy can reverse the biological effects of aging. HGH is widely appreciated for aesthetics and body recomposition, not necessarily as a performance enhancer.

HGH is released in pulses that take place during the day. Growth hormone is rapidly converted in the liver to its powerful growth promoting metabolite, Insulin like Growth Factor. IGF-1 is measured in the blood to determine the level of growth hormone secretion. Most of the beneficial effects of human growth hormone are directly attributable to IGF-1. Growth hormone replacement/stimulation is the most effective therapy to reverse the effects of aging.

At age 30 people have people have spent most of their lives with relatively high levels of HGH. HGH is responsible for growth during childhood as well as for repair and regeneration of tissue. By the time we are 30 our bodies no longer naturally produce enough GH to handle the damage that is taking place. As this continues, we age. Increasing HGH in our bodies, we can slow, or even reverse many of the manifestations of aging.
What HGH can do:

Reduce excess body fat, especially abdominal fat. (The reduction of abdominal fat is the single most profound effect of HGH replacement in many people)
Decrease in the waist to hip ratio. (Meaning fat is removed primarily from around the waist where it is associated with a high risk of coronary disease)
Increase muscle mass. (and physical strength if combined with moderate exercise)
Reduce wrinkling of the skin and some other effects of skin aging. (Compliments can be Melanotan and MT-II)
Re-grow certain internal organs that have atrophied with age.
Increase bone density.
Strengthen the immune system.
Enhanced feeling of well being.
Reverse cognitive decline.
Stimulate production of the bone marrow cells that produce red blood cells.
Reduce the probability that you will spend the last years of your life in a nursing home.
HGH slows the progression of cardiovascular disease, and reduces the risk of death from cardiovascular disease, in individuals with natural growth hormone levels that are below average for the age of the individual. HGH can also slow the progression of cardiovascular disease by improving one's cholesterol profile. There is increasing evidence over the past year or two that maintaining healthy growth hormone levels results in a stronger heart. Individuals with low growth hormone levels have an overall increased risk of death due to cardiovascular disease. Low growth hormone levels cause a particularly large increase in the risk of stroke as compared with individuals receiving growth hormone replacement.

HGH takes several weeks to months of use benefit from its effects. There are a number of substances that increase the natural secretion of HGH. Some of them are amino acids. The relationship of certain amino acids to growth hormone is complex and varies greatly among different individuals and among individuals of different ages. All absolute and universal statements made about this subject are clearly false.

HGH is a peptide that comes in the form of lyophilized powder. Any other form that you see advertised or run across is likely NOT the real deal. The only way to administer true HGH is by sub-q or intramuscular injection. Be aware that HGH is not anabolic by itself. Careful measures need to be in place during reconstitution. Oral Human Growth Hormone.

Reconstituting common HGH, one vial contains powdered freeze-dried HGH and the other vial contains sterile water with a bacteriostatic preservative. When the user is ready to begin, a certain amount of the sterile water is drawn out of the second vial (with a needle and syringe) and injected into the first vial to dissolve the powdered HGH. The solution is then ready for injection. The unused portion is to be kept refrigerated. Use reconstituted HGH within 3 or 4 weeks.

HGH peptide is dissolved by the user because HGH powder is much more durable. The dissolved HGH is very susceptible to being attacked by bacteria and degraded by enzymes. HGH is normally refrigerated, but if HGH powder is is left at room temperature for a few hours, no harm is done as long as the room is not too warm. Sterile powdered HGH can even be left in a cool room for days or weeks, but this is not a good idea. Even the powdered HGH can deteriorate if the temperature rises slightly above normal room temperature for any significant period of time.
The best way to begin HGH is to start with a low dose and ease your way into higher doses. This will allow you to avoid (or at least minimize) many of the more common sides of HGH such as bloating and joint pain. Most can tolerate 1-2 IU per day with few sides. HGH administration should be done on an empty stomach as fats and carbohydrates blunt release. Natural production of GH is 1 IU, give or take a half. For results in athletes the dosing should attempt to duplicate how the body releases the hormone naturally. Learning how much and when to best dose is very individual. My apologies for the dosing chart above.

For bodybuilders growth hormone is best taken in conjunction with insulin, anabolics/androgens, and thyroid hormone. Insulin is extremely effective with HGH as the injections cause a down regulation of insulin sensitivity in the body.

Half life of HGH is 2 hours when injected sub-q with a four hour period which there is a suppression of naturally produced GH. Intramuscular injections shorten the half life. Subcutaneous injections over the long term can lead to spot reduction. Whether or not HGH has any localized benefit from IM injection is unknown, but speculated.

HGH is tightly regulated by the FDA, but HGH is not a controlled substance on the federal level in the United States.

Growth Hormone Releasing Hormone (CJC-1295)
GHRH modified GRF 1-29 CJC-1295 Dose:
Light: 50mcg
Common: 100mcg
Large: 250mcg

Growth Hormone Releasing Hormone (GHRH) CJC-1295 is a synthetic secretalogue which stimulates HGH release. In the human body, large amounts of growth hormone are stored in the pituitary. GHRH affects the number of secreting cells and the amount they are able to secrete.

Dosing GHRH once at night for anti-aging purposes and multiple injections (1-3x) for anabolism. CJC-1295 has the ability to make the body produce its own GH as compared to using synthetic HGH. GHRP-6 use in conjunction with CJC-1295 is synergistic, amplifying the GH pulse considerably.

Researchers report positive effects much like that of HGH. Particularly energy, quality sleep, fat loss/metabolism, improved skin quality, pumps in the gym.

CJC-1295 is typically found in 2mg vials. Reconstitute with BW. Inject subcutaneously.

Most common desirable form to find and research with CJC-1295 (Growth Hormone Releasing Factor) is a modified GRF (1-29), lacking the DAC (drug affinity complex). This GHRH (mod GRF 1-29) has 30 minute half life, superior to that of Sermorelin. Post injection flush is common.

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